产品描述

UF010是I类HDAC选择性抑制剂，对HDAC1、HDAC2、HDAC3、HDAC8、HDAC6和HDAC10的IC50值分别为0.5 nM、0.1 nM、0.06 nM、1.5 nM、9.1 nM和15.3 nM。

靶点（IC50 & Targe）

HDAC

体外研究

In Cell-cycle analysis using MDA-MB-231 cells, UF010 mainly blocks G1/S transition with an increased G1 cell population and a reduced cell population in the S phase in a dose-dependent manner. UF010 has a half-life of 15.8 hr in cell culture medium containing 10% fetal bovine serum[1].

细胞实验

Cell lines: HCT116 cells and A549 cells

Concentrations: 2 μM

Incubation Time: 1 h

Method:HCT116 and A549 cells are exposed to DMSO or etoposide (Etop, 10 μM) for 6 hr. TSA (0.2 μM), MS-275, and UF010 (2 μM) are added 1 hr before cell lysis. The total cell lysates are subject to western blotting with antibodies to the indicated proteins. PCNA is detected as a loading control.

(Only for Reference)

参考文献

[1] Wang Y, et al. Chem Biol. 2015, 22(2):273-84.