分析实验室常备“武器”之HPLC衍生化试剂-百灵威

高效液相色谱（HPLC）是现代化合物分离鉴定的重要手段，然而，由于缺少通用检测器、样品被测组分含量低及其本身性质的限制，并非所有化合物都可直接用于高效液相色谱分析。衍生化反应可改变被测化合物性质，使之能够快速、灵敏、准确地被测定。

百灵威提供紫外、荧光等多种HPLC衍生化试剂。

常用紫外衍生化试剂

高效液相色谱大都配备紫外检测器，为使一些没有或有很弱紫外吸收的化合物能被检测，往往需要通过衍生化反应，在这些化合物分子中引入具有强紫外吸收的基团，使衍生物可被紫外检测器检测。

适用于羧基官能团检测

279588

99-73-0

2,4'-Dibromoacetophenone, 98%
2,4'-二溴苯乙酮

λmax: 254nm

434551

24463-19-2

9-Chloromethylanthracene, 98%
9-氯甲基蒽

λmax: 254nm

366373

70-11-1

2-Bromoacetophenone, 99%
2-溴苯乙酮

λmax: 254nm

254972

17564-64-6

N-(Chloromethyl)phthalimide, 97%
N-氯甲基邻苯二甲酰亚胺

λmax: 254nm

100792

5000-65-7

2-Bromo-3'-methoxyacetophenone, 98%, derivatization grade
2-溴-3'-甲氧基苯乙酮

λmax: 254nm

A5506

2978-11-2

N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea, 95.0%(LC)
N,N'-二异丙基-O-(4-硝基苯甲基)异脲

λmax: 254nm

A5503

54455-37-7

N-Chloromethyl-4-nitrophthalimide, 98.0%(T)
N-氯甲基-4-硝基邻苯二甲酰亚胺

λmax: 230nm

适用于氨基官能团检测

A5511

99-33-2

3,5-Dinitrobenzoyl Chloride, 99.0%(T)
3,5-二硝基苯甲酰氯

λmax: 254nm

A5512

70-34-8

2,4-Dinitrofluorobenzene, 99.0%(GC)
2,4-二硝基氟苯

λmax: 254nm

A5523

178065-29-7

Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide, 98.0%(LC)
Nα-(5-氟-2,4-二硝基苯基)-L-亮氨酰胺

λmax: 340nm

A5524

178065-30-0

Nalpha-(5-Fluoro-2,4-dinitrophenyl)-D-leucinamide, 98.0%(LC)
Nα-(5-氟-2,4-二硝基苯基)-D-亮氨酰胺

λmax: 340nm

A5513

103-72-0

Phenyl Isothiocyanate, 99.0%(GC)
异硫氰酸苯酯

λmax: 269nm

368359

68123-33-1

N-(4-Nitrophenylacetoxy)succinimide, 98%, derivatization grade, for HPLC Labeling
N-(4-硝基苯基乙酰氧基)琥珀酰亚铵

λmax: 254nm

210054

14152-97-7

2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate, 98%
2,3,4,6-四-O-乙酰基-β-D-葡萄糖异硫氰酸酯

λmax: 254nm

563716

132413-50-4

2,3,4,6-Tetra-O-benzoyl-β-D-glucopyranosyl isothiocyanate
2,3,4,6-四-O-苯甲酰基-β-D-吡喃葡萄糖基异硫氰酸酯

λmax: 254nm

适用于羟基官能团检测

A5511

99-33-2

3,5-Dinitrobenzoyl Chloride, 99.0%(T)
3,5-二硝基苯甲酰氯

λmax: 254nm

适用于羰基官能团检测

A5531

55907-61-4

2,4-Dinitrophenylhydrazine Hydrochloride, 98.0%(LC&T)
2,4-二硝基苯肼盐酸盐

λmax: 254nm

531712

2086-26-2

O-(4-Nitrobenzyl)hydroxylamine hydrochloride, 98%, derivatization grade
O-(4-硝基苄基)羟胺盐酸盐

λmax: 254nm

最大吸收波长：λmax
点击查看更多紫外衍生化试剂

常用荧光衍生化试剂

荧光检测器具有高灵敏度和高选择性，比紫外检测器的灵敏度高10‐1000倍。荧光衍生化试剂能与一些官能团产生强烈荧光，在较温和条件下与被测物质快速定量反应，尤其适合痕量组分分析。

适用于羧基官能团检测

A5554

139332-66-4

4-Nitro-7-piperazino-2,1,3-benzoxadiazole, 98.0%(LC&T)
NBD-PZ, 4-硝基-7-哌嗪-2,1,3-苯并恶二唑

λex: 470 nm; λem: 541 nm

418944

35231-44-8

4-Bromomethyl-7-methoxycoumarin, 97%
4-溴甲基-7-甲氧基香豆素

λex: 328 nm; λem: 380 nm

228839

88404-25-5

4-Bromomethyl-6,7-dimethoxycoumarin, 98%
4-溴甲基-6,7-二甲氧基香豆素

λex: 340 nm; λem: 425 nm

A5553

124522-09-4

3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one, 98.0%(T)
3-溴甲基-7-甲氧基-1,4-苯并恶嗪-2-酮

λex: 355 nm; λem: 430 nm

A5560

143112-50-9

(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(S)-(+)-DBD-Apy, (S)-(+)-4-(N,N-二甲氨基磺酰)-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑

λex: 470 nm; λem: 580 nm

A5561

143112-49-6

(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(R)-(-)-DBD-Apy, (R)-(-)-4-(N,N-二甲基氨基磺酰基)-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑

λex: 470 nm; λem: 580 nm

A5562

143112-52-1

(S)-(+)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(S)-(+)-NBD-Apy, (S)-(+)-4-硝基-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑

λex: 470 nm; λem: 540 nm

A5563

143112-51-0

(R)-(-)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(R)-(-)-NBD-Apy, (R)-(-)-4-硝基-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑

λex: 470 nm; λem: 540 nm

A5555

139332-64-2

4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole, for HPLC Labeling
DBD-PZ, 4-(N,N-二甲氨基磺酰基)-7-哌嗪-2,1,3-苯并恶二唑

λex: 440 nm; λem: 569 nm

A5573

221263-97-4

4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole, 95.0%(LC)
NBD-CO-Hz, 4-(N-肼羰甲基-N-甲氨基)-7-硝基-2,1,3-苯并恶二唑

λex: 475 nm; λem: 530 nm

A5574

189373-41-9

4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole
DBD-ED, 4-(N,N-二甲氨基磺酰)-7-(2-氨基乙基氨基)-2,1,3-苯并恶二唑

λex: 450 nm; λem: 560 nm

A5576

254973-02-9

4-Acetamido-7-mercapto-2,1,3-benzoxadiazole, 95.0%(LC)
AABD-SH, 4-乙酰氨基-7-巯基-2,1,3-苯并恶二唑

λex: 368 nm; λem: 524 nm

434551

24463-19-2

9-Chloromethylanthracene, 98%
9-氯甲基蒽

λex: 365 nm; λem: 412 nm

适用于氨基官能团检测

A5558

156153-43-4

4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole, 93.0%(T)
DBD-COCl, 4-(N,N-二甲基氨磺酰)-7-(N-氯甲酰甲基-N-甲氨基)-2,1,3-苯并恶二唑

λex: 450 nm; λem: 560 nm

A5595

98358-90-8

4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, 98.0%(LC)
DBD-F, 4-(N,N-二甲氨基磺酰)-7-氟-2,1,3-苯并恶二唑

λex: 450 nm; λem: 590 nm

A5569

163927-32-0

(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole, for HPLC Labeling
(S)-(+)-DBD-Py-NCS, (S)-(+)-4-(N,N-二甲氨基磺酰基)-7-(3-异硫氰酸基四氢吡咯-1-基)-2,1,3-苯并恶二唑

λex: 460 nm; λem: 550 nm

A5565

150993-62-7

(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(R)-(+)-DBD-Pro-COCl, (R)-(+)-4-(N,N-二甲氨基磺酰基)-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑

λex: 450 nm; λem: 560 nm

A5564

150993-63-8

(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(S)-(-)-DBD-Pro-COCl, (S)-(-)-4-(N,N-二甲氨基磺酰基)-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑

λex: 450 nm; λem: 560 nm

A5568

163927-31-9

(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole, for HPLC Labeling
(R)-(-)-DBD-Py-NCS, (R)-(-)-4-(N,N-二甲氨基磺酰基)-7-(3-异硫氰酸基四氢吡咯-1-基)-2,1,3-苯并恶二唑

λex: 460 nm; λem: 550 nm

A5579

N/A

4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride, 97.0%(T)
4-(4,5-二苯基-1H-咪唑-2-基)苯甲酰氯盐酸盐

λex: 325 nm; λem: 430 nm

A5575

147611-81-2

4-(N,N-Dimethylaminosulfonyl)-7-isothiocyanato-2,1,3-benzoxadiazole
DBD-NCS, 4-(N,N-二甲基氨磺酰)-7-异硫氰酸基-2,1,3-苯并恶二唑

λex: 384 nm; λem: 520 nm

A5592

10199-89-0

4-Chloro-7-nitro-2,1,3-benzoxadiazole, 98.0%(T)
NBD-Cl, 4-氯-7-硝基-2,1,3-苯并恶二唑

λex: 460 nm; λem: 535 nm

301307

29270-56-2

4-Fluoro-7-nitrobenzofurazan, 98%
4-氟-7-硝基苯并-2-氧杂-1,3-二唑

λex: 470 nm; λem: 530 nm

A5566

159717-69-8

(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(R)-(+)-NBD-Pro-COCl, (R)-(+)-4-硝基-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑

λex: 470 nm; λem: 540 nm

A5567

159717-68-7

(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(S)-(-)-NBD-Pro-COCl, (S)-(-)-4-硝基-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑

λex: 470 nm; λem: 540 nm

A5577

163927-29-5

(R)-(-)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(R)-(-)-NBD-Py-NCS, (R)-(-)-4-(3-异硫氰酸基吡咯烷-1-基)-7-硝基-2,1,3-苯并恶二唑]

λex: 488 nm; λem: 590 nm

A5578

163927-30-8

(S)-(+)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination
(S)-(+)-NBD-Py-NCS, (S)-(+)-4-(3-异硫氰酸基吡咯烷-1-基)-7-硝基-2,1,3-苯并恶二唑

λex: 488 nm; λem: 590 nm

适用于羟基官能团检测

A5558

156153-43-4

4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole, for HPLC Labeling
DBD-COCl, 4-(N,N-二甲基氨磺酰)-7-(N-氯甲酰甲基-N-甲氨基)-2,1,3-苯并恶二唑

λex: 450 nm; λem: 560 nm

A5565

150993-62-7

λex: 450 nm; λem: 560 nm

A5564

150993-63-8

λex: 450 nm; λem: 560 nm

A5578

163927-30-8

λex: 488 nm; λem: 590 nm

A5567

159717-68-7

λex: 470 nm; λem: 540 nm

A5566

159717-69-8

λex: 470 nm; λem: 540 nm

A5579

N/A

4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride, for HPLC Labeling
4-(4,5-二苯基-1H-咪唑-2-基)苯甲酰氯盐酸盐

λex: 325 nm; λem: 430 nm

适用于羰基官能团检测

385727

504-02-9

1,3-Cyclohexanedione, 99%
1,3-环己二酮

λex: 325 nm; λem: 430 nm

276443

33008-06-9

Dansyl hydrazine, 98%
丹磺酰肼

λex: 340 nm; λem: 525 nm

A5556

131467-86-2

4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole, for HPLC Labeling
DBD-H, 4-(N,N-二甲氨基磺酰)-7-肼基-2,1,3-苯并恶二唑

λex: 450 nm; λem: 565 nm

205040

131467-87-3

4-Hydrazino-7-nitro-benzofurazan hydrazine adduct, 98%, derivatization grade
4-肼基-7-硝基苯并恶二唑肼

λex: 470 nm; λem: 550 nm

适用于巯基官能团检测

A5558

156153-43-4

λex: 450 nm; λem: 560 nm

A5595

98358-90-8

4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, for HPLC Labeling
DBD-F, 4-(N,N-二甲氨基磺酰)-7-氟-2,1,3-苯并恶二唑

λex: 450 nm; λem: 590 nm

A5568

163927-31-9

λex: 460 nm; λem: 550 nm

A5569

163927-32-0

λex: 460 nm; λem: 550 nm

612818

49759-20-8

N-(9-Acridinyl)maleimide, 98%
N-(9-吖啶基)马来酰亚胺

λex: 355 nm; λem: 465 nm

301307

29270-56-2

4-Fluoro-7-nitrobenzofurazan, 98%
4-氟-7-硝基苯并-2-氧杂-1,3-二唑

λex: 470 nm; λem: 530 nm

A5592

10199-89-0

4-Chloro-7-nitro-2,1,3-benzoxadiazole, for HPLC Labeling
NBD-Cl, 4-氯-7-硝基-2,1,3-苯并恶二唑

λex: 460 nm; λem: 535 nm

激发波长：λex；发射波长：λem
点击查看更多荧光衍生化试剂

分析实验室常备“武器”之HPLC衍生化试剂

常用紫外衍生化试剂

常用荧光衍生化试剂

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