图1 TPQLs荧光染料 ^[1]

图2 TPQL probes for accurate liver-development imaging (A and C) Two-photon images of H2S (A) and APN (C) in 2, 4, 6, 8 and 10-dpf zebrafish: the zebrafish were cultivated with TPQL-N3 (10 mM) and TPQL-APN (10 mM) for 1 h at 25C. lex = 810 nm, lem = 500–550 nm. Scale bar, 100 mm. (B and D) Inhibitor experiment of H2S (B) and APN (D). (E–G) Normalized fluorescence intensity of the fluorescence images (A)–(D). Error bar = RSD (n = 5). (H) Co-location experiment: images of 6-dpf transgenic zebrafish preincubated with TPQL-APN for 60 min. (H1) green channel for TPQL-APN, lex = 405 nm, lem = 500–550 nm. Scale bar, 100 mm. (H2) red channel for transgenic red fluorescent protein, lex = 560 nm, lem = 570–620 nm. (H3) overlay channel. (H4) local amplification of (H3). Scale bar, 20 mm. (I and J) Western blot analysis showing CBS (I) and APN (J) expression in 2, 4, 6, 8, and 10-dpf zebrafish. The CBS and APN relative abundance was normalized with actin-relative abundance. ^[1]